| Egyptian Journal of Archeological and Restoration Studies


EJARS (Established 2011)

EJARS Will Publish the Second Special Issue Under Title "Selected topics in Egyptology"

EJARS Will Publish multidisciplinary Approaches from Diagnostic to Conservation of Archeological building Materials

Volume 4 ,issue 2 | Summer and Autumn 2014 | Pages : 105-112

APPLICATION OF DNA TECHNIQUES FOR IDENTIFICATION OF FUNGAL COMMUNITIES COLONIZING BOOK OF EGYPT VOLUMES V PLANCHES ANTIQUITIES

Document Type: Original article

DOI: 10.21608/EJARS.2014.7265

pages: 105-112

Authors:
Afandy, A. 1 ; Taha, A. 2

1 Assoc. Prof. Heritage Resources Management & Tour Guidance dept., College of Tourism & Archaeology, King Saud Univ. Riyadh, KSA. Conservation department, faculty of archaeology, Cairo Univ.
2 Conservation dept., High Institute of Tourism, Hotel Management & Restoration, Alexandria.

Abstract:
This study represents both a study case about the fungi associated with a description of Egypt volumes V Planches Antiquities in the Central library of Islamic manuscripts in Cairo, were used for identification of isolates from these brownish area from a description of Egypt The plate method with a DNA was used for identification of fungi. The two internal transcribed spacers and the 5.8S gene (ITS1-5.8S- ITS2) from the nuclear ribosomal DNA were amplified. The results show that the most dominant fungi isolated from the examined old paper samples belong to Aspergillus spp. (niger – flavus – fumigates and – terreus). In the present study, culture-independent molecular methods were applied to identify fungi communities colonizing paper samples. Some of these fungi are cellulolytic species. Pure fungal strains were used to constitute a marker for further comparative investigations of a description of Egypt volumes V Planches Antiquities. This study represents both a study case about the fungi associated with a description of Egypt volumes V Planches Antiquities in the Central library of Islamic manuscripts in Cairo, were used for identification of isolates from these brownish area from a description of Egypt The plate method with a DNA was used for identification of fungi. The two internal transcribed spacers and the 5.8S gene (ITS1-5.8S- ITS2) from the nuclear ribosomal DNA were amplified. The results show that the most dominant fungi isolated from the examined old paper samples belong to Aspergillus spp. (niger – flavus – fumigates and – terreus). In the present study, culture-independent molecular methods were applied to identify fungi communities colonizing paper samples. Some of these fungi are cellulolytic species. Pure fungal strains were used to constitute a marker for further comparative investigations of a description of Egypt volumes V Planches Antiquities.

Keywords:
manuscripts; DNA; Fungal strains; Investigations; diagnosis

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